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For anal sacculectomy, the animals were placed in a padded perineal stand and positioned in sternal recumbency with the tail reflected over their dorsum and secured. A mosquito hemostatic forceps was inserted into the anal sac to grasp the apex of sac. The sac was then exteriorized its orifice. Once the sac was fully everted, the anal sac was simply excised by using a monopolar electrocautery surgical unit (Ethicon Endo-Surgery, Cincinnati, OH, USA). Amoxicillin (20 mg/kg, per oral, twice a day) was administered to all animals for 7 days after surgery to prevent postoperative infection.
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